Optimization of process parameters for melanin nanoparticles synthesised from Pseudomonas stutzeri (BTCZ 109) using OFAT method and its anticancer property evaluation

Abstract

Melanins are biological macromolecule with immense functionality synthesised by a wide spectrum of living organism. It is mainly synthesised by the oxidative polymerization of indolic and phenolic compounds through several enzymatic process. It has wide spread application in agriculture, cosmetic and therapeutic industry due to its various properties including antioxidation ability, UV protection efficiency and anticancer activity. Because of this wide range of application in different sectors, large scale production and commercialization attains enormous consideration. The present study deals with the effect of 12 different process parameters on melanin production viz., production media, incubation time, inoculum concentration, pH, temperature, agitation, carbon source, phosphate and magnesium source, CuSO4.5H2O, sodium chloride and L-tyrosine on melanin production by Pseudomonas stutzeri strain BTCZ 109 obtained from Arabian sea sediments was evaluated. After optimizing the important process parameters, the bacteria showed about [~] 4.65 fold increase in melanin production compared to unoptimized cultural conditions. The melanin optimized through this method was found to be nano sized. The Nano sized DOPA melanin in treating Skin cancer cell line SK ML28 which showed a dose-dependent activity with an IC50 value of 164 g/mL. All these results highlight the therapeutic efficiency of DOPA melanin Nano particle as promising bioactive molecule. HighlightsO_LIProcess parameter optimization for large scale production of Nano-melanin obtained from Pseudomonas stutzeri strain BTCZ 109 through one-factor-at-a-time method. C_LIO_LIOptimization of 12 different process parameters were carried out which led to an increase in yield of [~] 4.65 fold compared to unoptimized culture conditions. C_LIO_LIIts efficiency in treating skin cancer cell line SK ML28 was also detected. C_LI