Genome sequencing, de novo assembly and annotation of the commercially important bamboo, Bambusa tulda Roxb.

Abstract

Bambusa tulda Roxb., a member of the Bambusoideae subfamily, is an ecologically and commercially important plant resource widely distributed on the Indian subcontinent. Our study reports long-read PacBio HiFi sequencing and genome assembly of B. tulda. Flow cytometry analysis revealed its estimated genome size [~]3 Gb. The de novo genome assembly of B. tulda predicted 43 contigs, distributed across three subgenomes, with a total size of 1.37 Gb, contig N50 of 35.69 Mb, and BUSCO score 99%. Repetitive elements constitute 63.31% of the genome. Functional annotation predicted 56,890 protein-coding genes, constituting 19.44% of the genome. This high-quality draft genome assembly will serve as an invaluable resource for future studies on the life history traits, phylogenomic analysis, comparative genomics, and targeted genome modification for important trait improvement of B. tulda.