High resolution and quantitative imaging of the postmortem brain

Abstract

MRI of fixed tissue is an excellent way to study pathological changes caused by different diseases with great anatomical detail. It is, however, known that properties of tissue change with fixation. The aim of this study was to determine the variability of several quantitative MRI (qMRI) parameters in fixed brain tissue obtained from donors unaffected by neurological conditions and investigate the existence of quantitative parameters which vary little between specimens. We introduce a 3D method for high-resolution mapping of water content, T1 and T2* relaxation times and parameters characterising magnetisation transfer and apply it at 3T to 7 whole, fixed human brains (3 male, 4 female, aged between 47 and 79 years, mean age 67 years). The qMRI parameters determined include relaxation rates T1 and T2*, MT ratio and T1 and T2* after MT. From these we can further derive semiquantitative MT parameters such as the exchange rate (ktrans) and bound pool fraction (fbound). Correlations between these parameters are investigated. In addition, truly quantitative water content determined non-invasively with MRI is reported on whole human post mortem brains - to our knowledge, for the first time. Water content was found to have mean values of 73% for WM and 85% for GM with standard deviation below 2.5% over 7 brains, and thus a few percent units higher than in vivo (69% and 81%) and of comparable constancy.