Silver thiosulfate and Benzyladenine in combination with pruning additively feminizes cassava flowers and modulates transcriptome

Abstract

Cassava, a tropical storage-root crop, is a major source of food security for millions in the tropics. Cassava breeding however is hindered by the poor development of flowers and female flowers in particular, since flower development is strongly skewed towards male flowers. Our objectives were to test plant growth regulator and pruning treatments for their effectiveness in field conditions in improving flower production and fruit set in cassava. Pruning the fork type branches that arise at the shoot apex immediately below newly formed inflorescences stimulated inflorescence and floral development. The anti-ethylene silver thiosulfate (STS) also increased flower abundance. Both pruning and STS increased flower numbers without influencing sex ratios. In contrast, the cytokinin benzyladenine (BA) feminized flowers without increasing flower abundance. Combining pruning and STS treatments led to an additive increase in flower abundance; with the addition of BA, over 80% of flowers were females. This three-way treatment combination of pruning+STS+BA also led to an increase in fruit development. Transcriptomic analysis of gene expression in tissues of the apical region and developing inflorescence revealed that the enhancement of female flower development by STS+BA was accompanied by the downregulation in of several genes associated with repression of flowering, including Tempranillo 1 (TEM1), GA receptor GID1b, and ABA signaling genes ABI1 and PP2CA. We conclude that treatments with pruning, STS and BA create widespread changes on the network of hormone signaling and regulatory factors beyond ethylene and cytokinin.