Phenotypic integration of post-germination traits in Quercus suber: morphological development is mediated by acorn mass; leaf physiology by populations aridity.

Abstract

Background and AimsAssessing intra-specific trait covariation across populations is essential to understand species adaptive responses to climatic variation. However, in tree species, this is understudied for early-life stages despite they are more vulnerable to environmental changes and that climatic adaptations can differ between tree ages. In this paper, we studied the integrated phenotype of Quercus suber during the months following germination. For that, we studied the covariation of key traits involved in seedlings water and C economies along a gradient of aridity at seed origin. MethodsWe performed a provenance trial with 157 Q. suber seedlings originating from 7 different populations across the species distribution. The seedlings were germinated and grown during 4 months under common conditions. Acorn mass along with 11 above- and below-ground traits involved in water and C use were measured. Their covariation in response to aridity at seed origin was analyzed using structural equation modelling (SEM). The variation of individual traits to increasing aridity and the mediation of acorn mass was also tested. Key ResultsSeedlings from arid populations displayed higher leaf evaporative demand coupled with a greater root water uptake capacity. Their leaf physiology also depicted a greater C acquisition capacity, strongly linked to traits conferring drought and heat tolerance. The development of above- and below-ground tissues responded mainly to acorn mass, whereas leaf physiology variations were associated to populations aridity. ConclusionsDry-origin seedlings display a more acquisitive strategy at the whole-plant level compared with seedlings from mesic provenances. This allows a greater water and carbon uptake capacities following germination, which is critical for their survival during their first summer. Leaf physiology adjustments to populations climate contrasts with its plasticity observed by other studies addressing juvenile trees, highlighting Q. suber varying adaptive strategies at different life stages.