Colorectal adenoma presence is associated with decreased menaquinone pathway functions in the gut microbiome of patients undergoing routine colonoscopy

Abstract

BackgroundColorectal cancer (CRC) is among the most common malignancies worldwide, with colorectal adenomas recognized as well-established precursors to CRC. Changes in gut microbiota appear to be linked to CRC by promoting chronic inflammation, immune dysfunction, and metabolic issues that drive tumor growth and progression. ObjectivesTo explore the relationship between gut microbiome composition and the presence of colorectal adenomas in patients undergoing routine colonoscopy. Materials and methodsPatients were selected from those receiving standard colonoscopy based on strict inclusion and exclusion criteria, to minimize potential confounding factors such as previous colorectal surgeries, inflammatory bowel diseases, and the use of antibiotics or probiotics. Fecal samples were collected before bowel preparation for the colonoscopy procedure, and metagenomic shotgun sequencing was used to analyze the composition and functions of the gut microbiome. ResultsOverall, 136 participants were recruited, and 56 of them had colorectal adenomas. Although no distinction was observed in alpha diversity, beta diversity analysis indicated significant differences between the adenoma-positive and adenoma-negative groups. Signs of dysbiosis were found in patients with adenomas: increased abundance of the genera Bacteroides and Prevotella and decreased abundance of Faecalibacterium and Anaerostipes species. Beta diversity analysis showed statistically significant differences in the structure of the microbiota. Significant differences in the relative abundance of UBA7597 sp003448195 were observed between groups. Functionally, decreased vitamin K2, SCFA (propionate) synthesis, along with lower Stickland fermentation activity was observed, indicating altered microbial metabolism. These changes may compromise epithelial barrier support, anti-inflammatory signaling, and energy metabolism in the colon. ConclusionsThe discovery of microbial taxa and functional pathways associated with the presence of adenomas underscores the potential of microbiota-based biomarkers and therapeutic strategies in the prevention and management of colorectal cancer.