The complete mitochondrial genome of Heikeopsis japonica (Decapoda: Brachyura: Dorippidae): Phylogenetic implications of the family Dorippidae

Abstract

Characterizing complete mitochondrial genome (mitogenome) supports comprehensive understanding in gene arrangement, molecular evolution and phylogenetic status. Previously, no studies have been conducted on the complete mitogenomes of the Dorippidae family within infraorder Brachyura. Herein, we firstly determined the sequence of Heikeopsis japonica (Decapoda: Brachyura: Dorippidae) mitogenome by high-throughput sequencing. Its mitogenome sequence is 15,980 bp in size, with 13 protein-coding genes, two ribosomal RNA genes, and 22 transfer RNA genes. The genome has a high A + T content of 73.52%, and low G + C content of 26.48%. The AT-skew is negative, -0.0079; and the GC-skew is positive, 0.322. The phylogenetic analysis of H. japonica using 40 Brachyuran mitogenome datasets indicated that Pyrhila pisum of family Leucosiidae had the closet relationship with H. japonica. Further selection pressure analysis of H. japonica suggested the presence of strong positive purification selection in Brachyuran. Meanwhile, a total of 31 residues located in genes atp6, atp8, cox1-3, cob, nad1-5, and nad4L were detected as the positively selected sites. This study reports the first available complete mitogenome of family Dorippidae, and our results could offer a useful phylogenetic implication of Dorippidae in the infraorder Brachyura.