The Enhanced Activation of Innate Immunity in Drosophila S2 Cells by Micrococcus luteus is Mediated by Relish

Abstract

The IMD and Toll signaling pathways in Drosophila melanogaster mediate the innate immune responses to Gram-negative and Gram-positive bacteria and fungi, respectively. Here we studied the involvement of the NF-{kappa}B transcription factor Relish, which is a mediator of the IMD pathway, in the humoral immune response to the Gram-positive bacteria Micrococcus luteus and Bacillus subtilis and the entomopathogenic fungus Metarhizium anisopliae, using D. melanogaster S2 cells as a model. Activation of Relish proteolysis was observed after S2 cell treatment with the control Gram-negative bacterium Escherichia coli. We found that M. luteus had also a noticeable effect on Relish activation, while B. subtilis and M. anisopliae effects were modest. Activation patterns of the genes encoding predominantly the IMD-pathway-dependent antimicrobial peptides (AMPs) and peptidoglycan recognition proteins (PGRPs), as well as the levels of Relish recruitment to the promoters of the genes, were found to be very similar in S2 cells treated with E. coli or M. luteus but were lower and differed in the case of B. subtilis and M. anisopliae. A Relish knockdown (KD) decreased the induction levels observed for all AMP and some PGRP genes in response to M. luteus treatment and the induction levels observed for several AMP genes after M. anisopliae and B. subtilis exposures. Therefore, our findings suggest that Relish plays a critical role in inducing the humoral immune response in Drosophila S2 cells, contributing primarily to the response against M. luteus and, to a lesser extent, to the responses against B. subtilis and M. anisopliae.