Ecological effects of genome size in yellow starthistle (Centaurea solstitialis) vary between invaded and native ranges

Abstract

Invasive species have become a pervasive threat on every continent and across a broad array of environments. Several traits predicted to promote invasion success, such as small seed size, rapid vegetative growth and short time to reproduction, are correlated with smaller genome sizes in a number of systems. To understand the influence of genome size on plant invasion dynamics, we compared genome sizes and traits in Centaurea solstitialis (YST) genotypes from the Californian invasion to those from their native source region in Spain. We conducted a common garden experiment and genome size survey to ask: (1) Is the invasion associated with genome size reduction? (2) To what extent can differences in genome size explain previously observed increases in plant size and reproduction in YST invasions? (3) Finally, we tested for expected evolutionary patterns in genome size across populations, including evidence of selection favouring reduced genome sizes at higher elevations, and evidence of stochastic processes leading to increases in genome sizes where effective population sizes are smaller. We found a reduction in corrected genome size in the invaded range, as well as significant interaction effects of range x elevation on genome size, and range x genome size on flowering time variation. Specifically, larger genomes tended to flower later and genome size decreased with increasing elevation in the invasion only. These emergent relationships in invading YST suggest potential selection for smaller genomes following introduction of YST to its invaded range. We also found a significant effect of measurement date on genome size estimation by flow cytometry, and this effect was more pronounced among native range genotypes.