Kin-recognition shapes collective behaviors in the cannibalistic nematode Pristionchus pacificus

Abstract

Kin-recognition is observed across diverse species forming an important behavioral adaptation influencing organismal interactions. In most species, proximate level mechanisms are poorly characterized, but in the nematode Pristionchus pacificus molecular components regulating its kin-recognition system have been identified which determine its predatory behaviors. This ability prevents the killing of kin however, its impact on other interactions including collective behaviors is unknown. Utilizing pairwise aggregation assays between distinct strains of P. pacificus, we observed aggregation between kin but not distantly related con-specifics. In these assays, only one strain aggregates with solitary behavior induced in the rival. Abolishing predation through Ppa-nhr-40 mutations results in rival strains successfully aggregating together. Additionally, interactions between P. pacificus populations and Caenorhabditis elegans are dominated by P. pacificus which also disrupts C. elegans aggregation dynamics. Thus, aggregating strains of P. pacificus preferentially group with kin, revealing competition and nepotism as previously unknown components influencing collective behaviors in nematodes.