Growth regulation bringing modularity to morphogenesis of complex three-dimensional exoskeletons

Abstract

Diverse three-dimensional morphologies of arthropods appendages, including beetle horns, are formed through the non-uniform growth of epidermis. Prior to moulting, epidermal tissue peels off from the old cuticle and grows non-uniformly to shape protruding appendages, which are often branching, curving, or twisting, from the planar epidermis. This non-uniform growth is possibly regulated by the distribution of morphogens on the epidermal cell sheet. Previous studies have identified molecules and signalling pathways related to such morphogenesis; however, how local regulation of cell sheet growth can transform planar epidermis globally into complex three-dimensional structures, such as beetle horns, remains unclear. To reveal the relationship between epidermal growth regulation and generated structures, this study theoretically examined how various shapes can be generated from planar epidermis under a deductive growth model that corresponds morphogen distributions to non-uniform growth on tissue. The results show that the heterochronic expression of multiple morphogens can flexibly fuse multiple simple shapes to generate various structures emulating complex appendages of beetles. These findings indicate that morphogenesis through such a mechanism may have developmental stability and modularity, providing insights into the evolution of the diverse morphology of arthropods.