ACS OmegaBioinformaticsPeer-reviewed journal article
Draft genome of the liver fluke Fasciola gigantica
Tripti Pandey, Arpita Ghosh, Vivek Nagaraj ToduriD, Vijayakumar RajendraniD, Parismita KalitaiD, Jupitara Kalita, Rohit ShuklaiD, Purna Bahadur ChetriiD, Harish ShuklaiD, Amit Kumar Sonkar, Denzelle Lee Lyngdoh, Radhika SinghiD, Heena Khan, Joplin Nongkhlaw, Kanhu Charan Das, Timir TripathiiD
Abstract
Fascioliasis is a neglected food-borne disease caused by liver flukes (genus Fasciola) and affects more than 200 million people worldwide. Despite technological advances, little is known about the molecular biology and biochemistry of the fluke. We present the draft genome of Fasciola gigantica for the first time. The assembled draft genome has a size of ~1.04 Gb with an N50 of 129 kb. A total of 20,858 genes were predicted. The de novo repeats identified in the draft genome were 46.85%. In pathway analysis, all the genes of glycolysis, Krebs cycle and fatty acid metabolism were found to be present, but the key genes for fatty acid production in fatty acid biosynthesis were missing. This indicates that the fatty acid required for the survival of the fluke may be acquired from the host bile. The genomic information will provide a comprehensive resource to facilitate the development of novel interventions for fascioliasis control.
- organism: virus
- assay: whole-genome sequencing
- research focus: genome assembly
- approach: statistical model
- Citations
- 31
- FWCI
- 5.98
- Field percentile
- top 2%
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