Morphology and Blood Metabolites Reflect Recent Spatial Differences Among Lake Winnipeg Walleye, Sander vitreus

Abstract

The invasive rainbow smelt (Osmerus mordax) was an abundant food source for Lake Winnipeg walleye (Sander vitreus), especially in the north basin of the lake, until the smelts collapse in approximately 2013. We quantified changing length-at-age ({approx} growth rates) and relative mass ({approx} body condition) in Lake Winnipeg walleye caught for a gillnet index data set. Here, walleye showed smaller length-at-age, particularly in the north basin with young fish, over time. This approach to assessing growth suggests a constraint in the north basin fish, possibly a nutritional limitation between 2017 and 2018, that was not present in the south. We then analyzed a separate group of walleye ([≥]452 mm in fork length) sampled in 2017 as part of a large-scale tracking study, which had a similar slope in length-mass relationship to large walleye caught in that year for the gillnet index data. A panel of metabolites associated with amino acid metabolism and protein turnover was compared in whole blood. These metabolites revealed elevated essential amino acids and suggest protein degradation may be elevated in north basin walleye. Therefore, based on both growth estimates and metabolites associated with protein balance, we suggest there were spatially distinct separations affecting Lake Winnipeg walleye with decreased nutritional status of walleye in the north basin of Lake Winnipeg being of particular concern.