Predicting plant biomass accumulation from image-derived parameters

Abstract

Image-based high-throughput phenotyping technologies have been rapidly developed in plant science recently and they provide a great potential to gain more valuable information than traditionally destructive methods. Predicting plant biomass is regarded as a key purpose for plant breeders and ecologist. However, it is a great challenge to find a suitable model to predict plant biomass in the context of high-throughput phenotyping. In the present study, we constructed several models to examine the quantitative relationship between image-based features and plant biomass accumulation. Our methodology has been applied to three consecutive barley experiments with control and stress treatments. The results proved that plant biomass can be accurately predicted from image-based parameters using a random forest model. The high prediction accuracy based on this model, in particular the cross-experiment performance, is promising to relieve the phenotyping bottleneck in biomass measurement in breeding applications. The relative contribution of individual features for predicting biomass was further quantified, revealing new insights into the phenotypic determinants of plant biomass outcome. Whats more, the methods could also be used to determine the most important image-based features related to plant biomass accumulation, which would be promising for subsequent genetic mapping to uncover the genetic basis of biomass.\n\nOne-sentence SummaryWe demonstrated that plant biomass can be accurately predicted from image-based parameters in the context of high-throughput phenotyping.\n\nFootnotesThis work was supported by the Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), the Robert Bosch Stiftung (32.5.8003.0116.0) and the Federal Agency for Agriculture and Food (BEL, 15/12-13, 530-06.01-BiKo CHN) and the Federal Ministry of Education and Research (BMBF, 0315958A and 031A053B). This research was furthermore enabled with support of the European Plant Phenotyping Network (EPPN, grant agreement no. 284443) funded by the FP7 Research Infrastructures Programme of the European Union.