Pseudo batch transformation: A novel method to correct for mass removal through sample withdrawal of fed-batch fermentations

Abstract

SummaryWe present a novel "pseudo batch" transformation algorithm that maps analytical data obtained for fed-batch bioreactor cultivations onto a constant volume batch process, significantly decreasing the complexity of characterizing the fed-batch process. Availability and implementationOur method is implemented in both Excel and Python and is available with tutorials and example data from https://github.com/biosustain/pseudobatch. The Python package is also available on PYPI under the name "pseudobatch". ContactLars Keld Nielsen, e-mail: lars.nielsen@uq.edu.au Supplementary informationA comprehensive explanation of the simulated fed-batch, parameter estimation procedures, and the Bayesian model can be found in supplementary information (S1-S5).