Host genotype shapes root mycobiota in durum wheat

Abstract

O_LIIn addition to environmental factors, plant genetics play a key role in shaping the root microbiota; however, the extent of this genetic control remains underexplored. Using a collection of 181 wheat lines derived from a genetically diverse population, we investigated the influence of wheat genotypes on the composition of the root endophytic mycobiota and explored the genetic determinants driving these relationships. C_LIO_LIWe first characterized the mycobiota associated with the roots of field-grown lines from the Evolutionary Prebreeding pOpulation (EPO) using Internal Transcribed Spacer 2 (ITS2) barcoding. Fungal diversity was then correlated with wheat genetics by quantitative methods, including heritability analysis and Genome Wide Association Studies (GWAS), to identify novel genetic determinants influencing the mycobiota composition. C_LIO_LIFungal species richness showed a positive correlation across most fungal clades, except between Mortierellomycotina and Glomeromycotina. Some specific fungal clades, such as Olpidiomycota or Chytridiomycota, underscored their potential as root endophytes. Additionally, we observed higher heritability in fungal clades (i.e. at the phylum or subphylum rank) that exhibit a homogenous trophic mode, such as the biotrophic Arbuscular Mycorrhizal Fungi (AMF). C_LIO_LIThis study identifies 11 QTLs associated with mycobiota composition at the clade level. By shedding light on the genetic control of fungal diversity and uncovering key fungal associations, this work enhances our understanding of plant-microbiota interactions and highlights the potential for breeding strategies to optimize these relationships. C_LI