Extrachromosomal DNA-mediated glutamine synthetase 2 (GS2) amplification enables glufosinate resistance in Amaranthus palmeri

Abstract

BackgroundPalmer amaranth achieves resistance to glufosinate by overproducing the chloroplastic glutamine synthetase (GS2) protein, a result of the amplification and overexpression of its nuclear coding gene. This study examined how amplified GS2 copies are inherited, identified their physical location in the cell, and investigated the mechanism of GS2 amplification. ResultsSegregation analysis revealed that inheritance of amplified GS2 copies deviates from classical Mendelian patterns, with poor correlation between plant level resistance and GS2 amplification. Fluorescence in situ hybridization revealed chromosomal insertions of GS2 and potential extrachromosomal circular DNA (eccDNA), and variability in GS2 amplification both among individual plants and within cells (not all cells in plants with high GS2 copy number showed GS2 amplification). The unpredictable inheritance patterns and distribution of GS2 copies across multiple chromosomes suggest a role for eccDNA in GS2 amplification. This was confirmed through eccDNA sequencing, which also identified multiple isoforms of GS2. ConclusionThis is the second documented case of herbicide resistance conferred by eccDNA-mediated target-site gene amplification in Palmer amaranth.