A web bench for analysis and prediction of oncological status from proteomics data of urine samples

Abstract

Urine-based cancer biomarkers offer numerous advantages over the other biomarkers and play a crucial role in cancer management. In this study, an attempt has been made to develop proteomics-based prediction models to discriminate patients of oncological disorders related to urinary tract and healthy controls from their urine samples. The dataset used in this study was obtained from human urinary peptide database that contains urine proteomics data of 1525 oncological and 1503 healthy controls with the spectral intensity of 5605 peptides. First, we identified peptide spectra using various feature selection techniques, which display different intensity and occurrence in oncological samples and healthy controls. Based on selected 173 peptide-based biomarkers, we developed models for predicting oncological samples and achieved maximum accuracy of 91.94% with 0.84 MCC. Prediction models were also developed based on spectral intensities with known peptide sequences. We also quantitated the amount of protein in a sample based on intensities of its fragments/peptides and developed prediction models based on protein expression. It was observed that certain proteins and their peptides such as fragments of collagen protein are more abundant in oncological samples. Based on this study, we also developed a web bench, CancerUBM, for mining proteomics data, which is freely available at http://webs.iiitd.edu.in/raghava/cancerubm/.