The transcription factor NO TRANSMITTING TRACT / WIP2 regulates cytokinin homeostasis in Arabidopsis

Abstract

NO TRANSMITTING TRACT / WIP2 (NTT/WIP2) is a zinc finger transcription factor that alters developmental programs in Arabidopsis when overexpressed. Loss of NTT function, alone or in combination with closely related genes, leads to developmental defects in gynoecia and roots, respectively. Its overexpression phenotype resembles the effects of cytokinin treatments, suggesting a potential connection to this hormonal pathway. To further investigate this connection, we conducted cytokinin content and response evaluations, and transcriptome analyses in an inducible NTT line. Alterations in cytokinin content and response, as evaluated with the TCSn::GFP line, were observed. In both cases, as in the global expression analyses, the observed changes were dependent on the tissue analyzed. Moreover, Y1H and transactivation assays suggested direct NTT binding to the IPT5, AHP6 and CKX7, regulatory regions. We propose that NTT, among other mechanisms, regulates development through the modulation of cytokinin homeostasis at different levels, including its local concentration. Since NTT is a target of AUXIN RESPONSE FACTOR 5 / MONOPTEROS, the results presented here indicate that NTT connects an auxin input to a change in cytokinin levels.