CCR3 antagonist impairs estradiol induced eosinophil migration to the uterus in ovariectomized mice

Abstract

The eosinophils in the uterus was associated with the successful implantation of the embryo. In addition, they are prevalent in the female reproductive tract, where they may contribute to remodeling and maintenance of epithelial integrity. However, the mechanisms by which eosinophils migrate into the uterus are still not entirely clear, since there are a variety of chemotactic factors that can cause migration of these cells. Therefore, to evaluate the CCR3 participation on eosinophil migration, we using ovariectomized C57BL/6 mice treated with CCR3 antagonist SB328437 and 17-{beta}-estradiol (E2). Thus, it was possible to confirm the hypothesis that the CCR3 receptor has an important role in the migration of these cells to the uterus of mice, because it was clearly observed histologically that the antagonist reduces this migration and activity eosinophil peroxidase (EPO) corroborated this result. Concomitant to this, there was a 46% reduction in uterine edema, as demonstrated by the uterus weight and histology. Together, these data suggest that the CCR3 is an important receptor involved in the eosinophils regulation in uterus, including influencing the uterine edema.