ELIP is preferentially expressed in stomatal guard cells and has a role in stomatal opening upon transition to light

Abstract

Plant early light-induced proteins (ELIPs) are chlorophyll-binding thylakoid membrane proteins commonly believed to function in photoprotection. However, results of studies with mutants or transgenic plants have been contradictory regarding this function, and the mechanistic workings of ELIPs have mostly remained elusive. We studied: (1) ELIP function in tomato (Solanum lycoperiscum) by generation and photosynthetic/physiological characterization of slelip mutants under various light conditions; and (2) ELIP tissue/cell localization by monitoring expression of the Venus yellow fluorescent protein fused to the native SlELIP1 promoter ( pSlELIP1::Venus) in transgenic tomato. Notably, slelip mutants did not display compromised photosynthetic performance and were not more sensitive to photoinhibition compared to parental plants, even following direct sunlight exposure. Surprisingly, pSlELIP1::Venus fusions revealed preferential expression in the leaf epidermis and specifically in stomatal guard cells, with no apparent expression in the mesophyll. No major alterations were observed in leaf gas exchange in slelip mutants at different light conditions. Intriguingly, stomatal conductance was reduced in slelip mutants upon the transition from dark to light. We propose an alternative hypothesis for plant ELIPs as players in the response of guard cells to light, connecting chlorophyll to stomatal function, and presenting a novel research direction for these enigmatic proteins.