Human-Dog Interaction Method and Dog Familiarity Differentially Modulate Prefrontal Connectivity and Autonomic Recovery Following Acute Stress: An fNIRS Study

Abstract

Human-dog interaction is widely used to alleviate stress, yet the accompanying cortical and autonomic dynamics during acute stress and recovery remain incompletely characterized. In this study, 70 adult dog owners completed a standardized stress protocol while prefrontal cortex activity was continuously monitored with functional near-infrared spectroscopy (fNIRS), alongside subjective stress and salivary cortisol measures. Participants then underwent a recovery phase involving interaction with a companion dog, manipulating contact type (direct in person vs. indirect video conferencing), and familiarity (own vs. unfamiliar dog). Stress responses were quantified through heart rate (HR), heart rate variability (HRV), low- and high-frequency spectral power (LF, HF, and LF/HF), and prefrontal functional connectivity (FC) based on maximum cross-correlation coefficients between fNIRS channels. As expected, HR, HRV-derived indices, and FC increased from baseline to the stress phase, confirming robust engagement of autonomic and prefrontal networks. During the recovery phase, all dog interaction conditions demonstrated reductions in HR, LF/HF ratio, and FC toward or below baseline, consistent with physiological and neural stress recovery; direct interaction was associated with particularly pronounced parasympathetic enhancement and a drop in FC that fell significantly below baseline in some cases. Across groups, HRV, LF/HF, and FC were the most consistent predictors of subjective stress ratings, whereas associations with cortisol were limited. These findings suggest that human-dog interaction promotes coordinated autonomic and prefrontal recovery from acute stress, and that fNIRS-derived metrics might provide a marker of stress modulation that can distinguish high-cognitive load and low cognitive demand states beyond traditional stress indices.