Develope Micro clonal-propagation protocol for Oxytenanthera abyssinica A. Rich. Munro to large scale micro-propagation

Abstract

In Ethiopia, Oxytenanthera abyssinica A.Rich. Munro has varies economic importance. However, conventional propagation methods of O. abyssinica are generally inefficient due to their low multiplication rate, time consuming, labor intensive, and too costly. The objective of this study was to develop a protocol for mass micropropagation of O. abyssinica through seed culture. Murashige and Skoog (MS) medium augmented with 6-Benzylaminopurine (BAP) was used for shoot initiation and multiplication. For in vitro rooting, MS medium supplemented with 3-Indole -butric acid (IBA) was used. In shoot initiation experiment all viable seeds were proliferated in 5-7 days of culturing. In shoot multiplication at 0.004 g/L BAP was Sucssefuly shoot multiplied, also best root responding were found at 0.005 g/l IBA. The present optimized protocol enables for any acters who needs large numbers of low land bamboo seedling for industery, small and micro enterprize or for reafforestation programms.