Life-history evolution under artificial selection in a clonal plant

Abstract

The response of natural populations to selection and the role of genetic correlations in constraining or facilitating evolutionary change is fundamental to adaptation. We use artificial selection to investigate the evolutionary response of clonal reproduction in the common monkeyflower (Mimulus guttatus), a species with extensive life history variation. We first characterize the standing genetic variation in a single perennial population, and then conduct four generations of divergent artificial selection on stolon number--the mechanism of clonal reproduction in this species. To start, stolon number had moderate heritability (H{superscript 2}=0.25) and was negatively genetically correlated with reproductive traits. Artificial selection produced a clear but asymmetrical response. High selection lines made significantly more stolons, while low lines diverged less from controls. Analyses of G matrices revealed that selection not only changed trait means but also genetic correlations, with high lines diverging more in multivariate genetic architecture. Our results demonstrate that single populations harbor sufficient genetic variation to respond rapidly to selection on clonality, and the response is shaped by existing patterns of genetic covariation. The capacity for rapid evolution of clonal traits is particularly relevant as climate change alters selection and shifts the relative advantages of sexual versus clonal life-history strategies.