Nest Ecology-associated Impacts of Wastewater on Wild Bee Microbiomes

Abstract

Anthropogenic pollution affects environments differently depending on proximity to pollution source, exposure route, and species ecology. Thus, understanding organisms ecological role and exposure route to contaminants is central to assessing pollution impact. Treated municipal wastewater releases contaminants into waterways and alters microbial communities. Plants absorb contaminants and expose animals through foraging and nest-building activities. Nesting ecology differences of ground vs wood cavity-nesting bees offers insight into niche-specific susceptibility to pollution. Because contaminants bind to soil strongly, ground-nesting bees near wastewater are likely most impacted, while wood cavity-nesting bees likely less impacted since plants ability to uptake contaminants are species dependent. We compared gut microbiomes of directly exposed soil-nesting Halictus ligatus and indirectly exposed wood-nesting Ceratina spp. upstream/downstream of wastewater. We collected bees, flowers, and soil, and analyzed their bacteria microbiomes (16S rRNA). Wastewater altered ground-nesting H. ligatus microbiome >18 times greater than wood cavity-nesting Ceratina adults. Ceratina larvae and pollen provisions showed significant but smaller shifts. Conversely, soil and flower microbiomes remained stable, indicating higher resilience. These results demonstrate that exposure routes drive contaminants susceptibility, with animal-associated microbes most vulnerable. Because bees are important pollinators and biodiversity contributors, these disruptions point to broader ecological risks in increasingly contaminated landscapes. O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=118 SRC="FIGDIR/small/675205v1_ufig1.gif" ALT="Figure 1"> View larger version (61K): org.highwire.dtl.DTLVardef@679a58org.highwire.dtl.DTLVardef@1c61700org.highwire.dtl.DTLVardef@101359org.highwire.dtl.DTLVardef@1664987_HPS_FORMAT_FIGEXP M_FIG C_FIG