Understanding Activity-Stability Tradeoffs in Biocatalysts by Enzyme Proximity Sequencing
R. Vanella, C. Kueng, A. A. Schoepfer, V. Doffini, J. Ren, M. Nash
The paper introduces EP-Seq, a method to simultaneously measure enzyme stability and activity across thousands of mutations, and applies it to D-amino acid oxidase to identify activity-stability tradeoffs.
bioRxiv · Feb 24, 2023Bioengineering