Cell-free mutant analysis combined with structure prediction of a lasso peptide biosynthetic protein B2
A. Alfi, A. Popov, A. Kumar, K. Y. J. Zhang, S. Dubiley, K. Severinov, S. Tagami
The paper combines cell-free mutagenesis with AlphaFold2 structure prediction to validate the function of TfuB2, revealing a hydrophobic binding site for TfuB1.
bioRxiv · Apr 6, 2022Biochemistry