Probing single cell fermentation flux and intercellular exchange networks via pH-microenvironment sensing and inverse modeling
V. Onesto, S. Forciniti, F. Alemanno, K. Narayanankutty, A. Chandra, S. Prasad, A. Azzariti, G. Gigli, A. Barra, A. De Martino, D. de Martino, L. L. del Mercato
This study quantifies single cell fermentation fluxes and intercellular exchange networks using pH microenvironment sensing and inverse modeling in mixed cancer and fibroblast cultures, revealing heterogeneous proton trafficking and a crossover from hub-driven to dipolar exchange phases.
bioRxiv · May 3, 2022 · PreprintBiophysics