Multiplexed competition in a synthetic squid light organ microbiome using barcode-tagged gene deletions
H. L. Burgos, E. F. Burgos, A. J. Steinberger, G. Suen, M. J. Mandel
The paper develops a method to rapidly generate barcode-tagged gene deletions in Vibrio fischeri and uses high-throughput competition experiments to identify colonization factors in the squid microbiome.
bioRxiv · Aug 24, 2020Microbiology