In vivo single-cell analysis using calcofluor - white staining detects high expression phenotype in L. lactis cultures engineered for hyaluronic acid production.
A. B. MUTHUKRISHNAN, G. Jayaraman, A. Hakkinen, V. D. Rajendran, A. Kozhiyalam
This paper uses calcofluor white staining and in vivo fluorescence confocal microscopy to detect high-expression phenotypes in L. lactis engineered for hyaluronic acid production, revealing significant heterogeneity in HA expression.
bioRxiv · Oct 21, 2020 · PreprintBioengineering