High sensitivity (zeptomole) detection of BODIPY heparan sulfate (HS) disaccharides by ion-paired RP-HPLC and LIF detection enables analysis of HS from mosquito midguts.
M. L. Maciej-Hulme, A. C. Leprince, A. Lavin, S. E. Guimond, J. E. Turnbull, J. Pelletier, E. A. Yates, A. K. Powell, M. A. Skidmore
This paper develops a highly sensitive method to detect BODIPY-labeled HS disaccharides, enabling analysis of HS from small biological samples like mosquito midguts.
bioRxiv · Jan 21, 2020 · PreprintBiochemistry