ORBIT for E. coli: Kilobase-scale oligonucleotide recombineering at high throughput and high efficiency
This paper introduces ORBIT, a high-throughput, high-efficiency method for creating kilobase-scale genomic modifications in E. coli using DNA oligonucleotides and Bxb-1 integrase targeting.
bioRxiv · Jun 28, 2023 · PreprintMicrobiology