CRISPRi screen for enhancing heterologous α-amylase yield in Bacillus subtilis
A. S. Geissler, A. O. Fehler, L. D. Poulsen, E. Gonzalez-Tortuero, T. B. Kallehauge, F. Alkan, C. Anthon, S. E. Seemann, M. D. Rasmussen, A. Breüner, C. Hjort, J. Vinther, J. Gorodkin
The paper uses CRISPRi to knock down non-coding RNA regions in Bacillus subtilis to identify those affecting α-amylase yield, finding some antisense RNAs increase or decrease yield.
bioRxiv · Mar 30, 2022Microbiology