genetic perturbation / CRISPR

EWSR1::ATF1 hyperactivation via TRIM11 depletion triggers lethal metabolic rewiring in clear cell sarcoma

This study identifies TRIM11 as a regulator of EWSR1::ATF1 stability in clear cell sarcoma, showing its depletion leads to metabolic rewiring and cell death, with PCK1 as a potential biomarker.

Gene-resolved metagenomics reveals partitioned nitrogen transformation potential in a landfill leachate sequencing batch reactor microbiome

This study uses gene-resolved metagenomics to analyze nitrogen transformation potential in a landfill leachate SBR microbiome, revealing gene distribution across taxa and potential nitrogen retention pathways.

KAT6 and KAT7 actively maintain mitotic chromatin composition and acetylation to preserve developmental competence

This study shows KAT6 and KAT7 maintain mitotic chromatin acetylation and composition, supporting postmitotic transcriptional memory and developmental competence in stem cells.

A TMEM175 Mutation Disrupts the Lysosomal-Mitochondrial Axis Causing Short QT Syndrome and Sudden Cardiac Death

This paper identifies a TMEM175 mutation causing short QT syndrome and sudden cardiac death by disrupting lysosomal-mitochondrial interactions and impairing mitochondrial function.

In situ single-cell CRISPR screens uncover context-dependent rewiring of glioma states and immune recruitment

The paper uses in vivo single-cell CRISPR screens to study how tumor suppressor loss and genetic perturbations affect glioma states and immune recruitment in mice.

Targeted Deletions Uncover Previously Uncharacterised C1-fixing Cluster Genes as Essential for Autotrophy in Clostridium autoethanogenum

The paper identifies essential C1-fixing cluster genes in Clostridium autoethanogenum using gene deletions and functional analysis, revealing their roles in autotrophy and metabolism.

SEC16A/MAG5-mediated ER export shapes ABA receptor homeostasis

The paper identifies SEC16A/MAG5 as essential for ABA receptor homeostasis by mediating ER export and trafficking, impacting ABA signaling without affecting ABA levels.

Cell cycle progression and stalked cell morphogenesis in Caulobacter crescentus are regulated independently by the flagellum

This study identifies fss genes that regulate stalked cell morphogenesis and cell cycle progression independently of the flagellum in Caulobacter crescentus.

SAMP1/TMEM201 protects alveolar type 2 cells from DNA damage-associated senescence and restrains pulmonary fibrosis

This study identifies SAMP1 as a protector of alveolar type 2 cells against DNA damage-induced senescence and shows its role in limiting pulmonary fibrosis in a mouse model.

Programmable interphyla communication for Staphylococcus aureus by transferability of homoserine lactone sensors between Bacillales bacteria

This study demonstrates the transferability of homoserine lactone sensors between Bacillales bacteria, enabling programmable interphyla communication between Gram-negative and Gram-positive species.

Sequential pathways preserve kinetochore integrity in meiosis

This study identifies three distinct pathways maintaining kinetochore integrity during meiosis in budding yeast, with distinct temporal requirements and interactions involving Ctf19cCCAN, Mif2CENP-C, and Cse4CENP-A-Okp1CENP-Q-Ame1CENP-U.

Druggable CSNK2A1-MeCP2 regulatory axis links Okur-Chung neurodevelopmental syndrome with MECP2-related disorders

The paper identifies a druggable CSNK2A1-MeCP2 regulatory axis linking Okur-Chung neurodevelopmental syndrome with MECP2-related disorders through CRISPR screens and mouse models.

Plumage diversity without differential expression of pigmentation genes in a rapid avian radiation

The paper investigates plumage diversity in Capuchino Seedeaters by analyzing gene expression and variants, finding that pigmentation differences arise from regulatory changes rather than differential gene expression.

Monounsaturated fatty acid depletion is a metabolic pattern of pathogenesis surveilled to activate p38 MAPK innate immunity

The paper identifies oleate depletion as a metabolic signal sensed by C. elegans to activate innate immunity against bacterial infection.

The long noncoding RNA FOSIL shapes the dynamics of FOS induction

This study shows that the lncRNA FOSIL regulates the timing and resolution of FOS induction by modulating chromatin structure and nuclear speckle dynamics.

Mapping the regulatory wiring of endothelial cell differentiation reveals a critical role for Hippo mechanosignaling

The paper maps gene regulators of endothelial cell differentiation using CRISPR and single-cell RNA-seq, identifying Hippo mechanosignaling as critical for endothelial identity.

Ferroptosis of selective progenitor cell types during embryogenesis ensures proper organ morphogenesis

This study identifies ferroptosis in embryonic lung and kidney progenitor cells, showing its role in organ morphogenesis through genetic manipulation of Gpx4.

RNA Polymerase Backtracking Promotes Type III-A CRISPR DNA Targeting

The paper shows RNA polymerase backtracking promotes type III-A CRISPR DNA targeting by inhibiting GreA anti-backtracking factors, which suppress Cas10-Csm activity.

A new Serratia taxon found in a trombidiform mite shows genomic evidence of host adaptation but is phylogenetically distinct from Serratia symbiotica

The paper identifies a new Serratia taxon in mites with genomic signs of host adaptation but phylogenetically distinct from S. symbiotica.

Genetic disruption of cutaneous de novo glucocorticoid synthesis: a CRISPR-Cas9 CYP11B1-knockout human epidermal model

This paper creates a CRISPR-Cas9 CYP11B1-knockout human epidermal model to study the effects of blocking cutaneous glucocorticoid synthesis.

Nutrient-responsive midgut turnover is spatially controlled by serotonin signaling

The paper shows serotonin signaling spatially controls nutrient-responsive midgut turnover in Drosophila by regulating enteroblast maturation and morphological changes through the 5HT7 receptor.

mTORC1-dependent control of Hedgehog signaling mediates nutrient-responsive licensing of intestinal stem cell differentiation

This study identifies an mTORC1-PWP1-Hedgehog circuit that links nutrient metabolism to intestinal stem cell differentiation in Drosophila, showing its role in aging and tissue renewal.

Apelin signaling controls neuroendocrine homeostasis via Atoh8-dependent transcriptional regulation

This study shows Apelin signaling regulates neuroendocrine homeostasis in zebrafish via Atoh8-dependent transcriptional control, affecting development, fertility, and aging.

Endogenous PROM1 activation redistributes liver cancer cells along a preexisting differentiation axis

The paper shows PROM1 activation in liver cancer cells shifts their state along a preexisting differentiation axis, suggesting CD133's role extends beyond being a stem-like marker.

DPYD links glial lipids and myelination to sleep duration

This study identifies DPYD's role in sleep regulation through glial lipid metabolism and myelination across species, linking genetic variants to oligodendrocyte-specific control.

LplA-mediated rescue of lethal myopathy and cardiomyopathy in mitochondrial lipoylation-deficient mammalian caused by Lipt1

This study uses AAV-delivered LplA to rescue lethal mitochondrial lipoylation defects in Lipt1-deficient mice, improving survival, motor function, and cardiac health.

Marked Mutants Allow for Rapid Non-Invasive Genotyping of Zebrafish by Visual Inspection Alone

This paper introduces a method to rapidly genotype zebrafish mutants using visual inspection by inserting fluorescent markers into target genes, enabling non-invasive identification of genotypes.

Proteolytic feedback of quorum sensing promotes infection by Pseudomonas aeruginosa

This study shows that proteolytic feedback regulates quorum sensing in Pseudomonas aeruginosa by controlling RhlR stability, affecting virulence and biofilm formation.

Biosensor-guided genome-wide CRISPR screens enable cis,cis-muconic acid overproduction in yeast

This study uses biosensor-guided CRISPR screens in yeast to identify genetic targets that enhance production of cis,cis-muconic acid and protocatechuic acid.

Accumulation of two distinct alleles causes astringency loss in cultivated polyploid persimmon

This study identifies the TAN gene as the causal factor for astringency loss in PCNA persimmon cultivars through haplotype-resolved forward genetics and long-read sequencing.