The energy landscape for R-loop formation by the CRISPR-Cas Cascade complex
D. J. Kauert, J. Madariaga Marcos, M. Rutkauskas, A. Wulfken, I. Songailiene, T. Sinkunas, V. Siksnys, R. Seidel
This paper uses ultrafast DNA unwinding experiments to study the energy landscape of R-loop formation by the Cascade complex, revealing a downhill bias followed by an uphill bias and showing base flips and mismatches modulate the landscape.
bioRxiv · Mar 17, 2023Biophysics