Beyond the Axenic Culture Filtrate Secretome: BONCAT and conventional proteomics together reveal the Infection-associated secreted protein repertoire of Acinetobacter baumannii in the human lung Epithelial cell line A549
Mohd Ilyas, Ruchi Tomar, Prabhakar Babele, Niti Singh, Pratibha Sharma, Shweta Tyagi, Yukti Gupta, Sneh Bajpai, Avika Kalra, Mahima Kaushik, Rahul Mahlawat, Saifullah Mohd, Pratiksha Pandita, Aastha Dua, Manjistha Baruah, Krishnamohan Atmakuri
This study uses BONCAT and conventional proteomics to identify infection-associated secreted proteins of Acinetobacter baumannii in A549 cells, revealing 152 proteins not detected in axenic cultures.
bioRxiv · Oct 28, 2026 · PreprintMicrobiology