PSF-Driven Spatio-Temporal Blending in Fluorescence Lifetime Imaging Microscopy and Its Mitigation via Mean-Shift Super-Resolution-Based Masking.
Mario González-Gutiérrez, Diana M. Vázquez-Enciso, Nicolas Mateos, Wonsang Hwang, Esley Torres, Haydee O. Hernández, Jenu Varghese Chacko, Iván Coto Hernández, Pablo Loza‐Álvarez, Christopher D. Wood, Adan Guerrero
This paper introduces a method to mitigate spatio-temporal blending in FLIM by using MSSR-based masking, improving spatial resolution while preserving lifetime accuracy.
ACS Photonics · Sep 14, 2026Peer-reviewedBiophysics