BiochemistryJournal of Proteome ResearchPeer-reviewed
Differences in Protein Capture by SP3 and SP4 Demonstrate Mechanistic Insights of Proteomics Clean-up Techniques
Jessica M. Conforti, Amanda M. Ziegler, Charli S. Worth…·Elyssia S. Gallagher
This study compares SP3 and SP4 proteomics clean-up techniques, finding SP3 captures hydrophilic proteins via HILIC-like and aggregation mechanisms, while SP4 captures hydrophobic proteins via aggregation.
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