Differences in Protein Capture by SP3 and SP4 Demonstrate Mechanistic Insights of Proteomics Clean-up Techniques
Jessica M. Conforti, Amanda M. Ziegler, Charli S. Worth, Adhwaitha M. Nambiar, Jacob T. Bailey, Joseph H. Taube, Elyssia S. Gallagher
This study compares SP3 and SP4 proteomics clean-up techniques, finding SP3 captures hydrophilic proteins via HILIC-like and aggregation mechanisms, while SP4 captures hydrophobic proteins via aggregation.
Journal of Proteome Research · Aug 20, 2024Peer-reviewedBiochemistry