Engineering a promiscuous pyrrolysyl-tRNA synthetase by a high throughput FACS screen
Adrian Hohl, Ram Karan, Anstassja Akal, Dominik Renn, Xuechao Liu, Alaguraj Dharamarajnadar, Seema Ghoprade, Michael Groll, Magnus Rueping, Jörg Eppinger
This study engineers a highly promiscuous PylRS variant using a single FACS screen, enabling aminoacylation of 31 lysine derivatives.
bioRxiv · Dec 5, 2017 · PreprintBiochemistry