Periplasmic production of Green Fluorescent Protein is poorly tolerated by Escherichia coli
Alexander Osgerby, Tim William Overton
This paper shows that periplasmic expression of GFP variants like sfGFP harms E. coli physiology, limiting their use as screening tools for recombinant protein production.
bioRxiv · Sep 28, 2025 · PreprintBioengineering