Author

Tim William Overton

University of BirminghamORCID 0000-0003-3050-2549Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

Click to filter author papers

Signature Assays, Organisms & Methods

Click to filter · or open tag

Research Focus:cell type annotation· 1 Organism:E. coli· 1 Assay:fluorescence / live-cell microscopy· 1 Approach:microscopy & imaging analysis· 1

Frequent Co-Authors

Alexander Osgerby· 1 shared

Activity by Year

2025-2025 · peak 1/yr

1 paper

Recent·Most cited

BioengineeringbioRxivPreprintSep 28, 2025

Periplasmic production of Green Fluorescent Protein is poorly tolerated by Escherichia coli

Alexander Osgerby, Tim William Overton

This paper shows that periplasmic expression of GFP variants like sfGFP harms E. coli physiology, limiting their use as screening tools for recombinant protein production.

DOI 10.1101/2025.09.28.679025

E. colifluorescence / live-cell microscopy

𝕏 PostBluesky