Expression and Purification of a Mammalian Protein: Cytosolic Domain of IRE1α from Insect Sf21 Cells
Amrita Oak, Grace Jansen, Christina Chan
The paper describes the expression and purification of the cytosolic domain of IRE1α in insect cells using a baculoviral system, achieving over 85% purity.
bioRxiv · Aug 30, 2019 · PreprintMolecular Biology