Engineering of CRISPR-Cas PAM recognition using deep learning of vast evolutionary data
Stephen Nayfach, Aadyot Bhatnagar, Andrey Novichkov, Gabriella O. Estevam, Nahye Kim, Emily Hill, Jeffrey A. Ruffolo, Rachel A. Silverstein, Joseph P. Gallagher, Benjamin P. Kleinstiver, Alexander J. Meeske, Peter Cameron, Ali Madani
This paper introduces Protein2PAM, a deep learning model that predicts CRISPR-Cas PAM specificity from protein sequences, enabling the design of Cas variants with tailored PAM recognition.
bioRxiv · Jan 6, 2025 · PreprintSynthetic Biology