Substitution of a valine to glutamic acid in the omega-like loop of MSMEG_6194 of Mycobacterium smegmatis interchanges its activity from DD-carboxypeptidase to beta-lactamase
Aditya Prasad Panda, Debasmita Chatterjee, Anik Roy, Sarmistha Biswal, Anindya S Ghosh
This study identifies MSMEG_6194 in Mycobacterium smegmatis as a DD-carboxypeptidase, showing that a single amino acid substitution converts its activity to beta-lactamase.
bioRxiv · Nov 20, 2025 · PreprintMolecular Biology