Disagreement between demultiplexing methods reveals structured cell quality gradients in multiplexed single-cell data
Ezgi Sen, Simon Steiger, Mislav Bašić, Nina Prokoph, Afzal P Syed, Isabelle Seufert, Usama‐Ur Rehman, Sabrina Schumacher, Anja Baumann, Michaela Feuring‐Buske, Niels Weinhold, Michael Lübbert, Hartmut Döhner, Konstanze Döhner, Marc Steffen Raab, , Karsten Rippe
This paper introduces Split-flow, a pipeline that uses concordance between demultiplexing methods to classify cell quality in multiplexed single-cell data, revealing structured quality gradients.
bioRxiv · May 13, 2026 · PreprintBioinformatics