Labeling of prokaryotic mRNA in live cells using fluorescent in situ hybridization of transcript-annealing molecular beacons (FISH-TAMB)
Rachel L. Harris, Maggie C. Y. Lau, Esta van Heerden, Errol Cason, Jan-G Vermeulen, Anjali Taneja, Thomas L. Kieft, Christina DeCoste, Gary Laevsky, Tullis C. Onstott
The paper develops FISH-TAMB to label living prokaryotic cells by hybridizing molecular beacons to target mRNAs, enabling study of rare microorganisms and their interactions.
bioRxiv · Aug 19, 2017 · PreprintMicrobiology