Mining the Proteome of Human Ovarian Cancer Extracellular Vesicles Using Thermolysin Proteolysis
Tyler T. Cooper, Jaihui Lui, Olena Bilyk, Michael Jewer, AOCS Study Group, Helen L. Steed, Yangxin Fu, Lynne-Marie M Postovit, Gilles A. Lajoie
This study compares Thermolysin and Trypsin/LysC proteolysis for enhancing proteomic analysis of ovarian cancer extracellular vesicles, finding Thermolysin improves peptide complexity and identifies unique proteins like Ly6E and NODAL.
bioRxiv · Oct 10, 2024 · PreprintCancer Biology