Analysis of SpCas9 on- and off-target effects in high efficiency multiplex editing in Arabidopsis
Johannes Stuttmann, Jens Keilwagen, Philippe Ortet, Fang Shiang Lim, Thomas Schmutzer, Christel Llauro, Assane Mbodj, Marie Mirouze, Frank Hartung, Ralf Wilhelm
The paper analyzes SpCas9 on- and off-target effects in Arabidopsis using high-efficiency multiplex editing and finds high specificity but potential for extensive on-target modifications at complex loci.
bioRxiv · Sep 29, 2026 · PreprintPlant Biology