Characterization of a replicating mammalian orthoreovirus with tetracysteine tagged μNS for live cell visualization of viral factories
L. Bussiere, P. Choudhury, B. Bellaire, C. L. Miller
This paper develops a method to visualize viral factories (VFs) in live cells by tagging the NS protein of mammalian orthoreovirus with a tetracysteine (TC) tag and using FlAsH-EDT2 reagent, revealing dynamic VF movement and microtubule dependence.
bioRxiv · Aug 9, 2017Microbiology